Overexpression Cell Line-Cell Culture-盟基生物科技股份有限公司

Cell line (穩定細胞株)

Overexpression Cell Line

Overexpression Cell Line

Cell line (穩定細胞株)

Overexpression Cell Line

一、產品介紹

細胞在藥物開發過程中扮演至關重要的角色,而有效產生目標重組蛋白和抗體的Stable Cell Line可以有效節省時間並降低成本。Biointron 使用 ECACC 許可的 CHO-K1 細胞進行細胞開發服務,其經驗豐富的團隊可快速生產高產量細胞株,以滿足客戶的基礎研究和CMC開發需求。

 

二、產品特色

1. 已經成功構建200多個Overexpression Cell Line。
2. 目標蛋白包含簡單的Single-Pass membrane Protein (例如:PD-L1 Protein) 和難度較大的 Multi-Pass membrane Protein (例如:G protein–coupled receptor, class C, group 5, member D (GPRC5D))
3. 陽性率達90%以上 (使用Flow cytometry檢測)

 
CHOK1 - hCTLA4 cell M3 Mean FITC-H Fold Change
CHOK1-hCTLA4 + anti-hFc 253 1
CHOK1-hCTLA4 + anti B6927+anti-hFc 153207 646.44
CHOK1+ anti B6927+anti-hFc 237 1
 
利用流式細胞儀確認 Human CTLA4 Cell line (CHO-K1) CTLA4表現的情況。
結果顯示: CHOK1-hCTLA4 cell line 其 CTLA4 (anti-B6927) 表現量較 WT 多 646.44倍
 
 
 
三、訂購資訊
 
Catalog Product Name Specificity Host Cell Growth/Thaw Medium Vector Delivery Size
B21583003 Human NaPi2B Cell line (CHO-K1) Human NaPi2B CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B21583004 Human GUCY2C Cell line (CHO-K1) Human GUCY2C CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B21630701 Human GPRC5D Cell line (CHO-K1) Human GPRC5D CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B21927402 Human CD137 Cell line (CHO-K1) Human CD137 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B21927403 Human CD28 Cell line (CHO-K1) Human CD28 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22131101 Human LRRC15 Cell line(CHO-K1) Human LRRC15 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22190801 Human ROR1 Cell line (CHO-K1) Human ROR1 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22301201 Human PD-L1 Cell line (CHO-K1) Human PD-L1 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22339901 Human CTLA4 Cell line (CHO-K1) Human CTLA4 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22339902 Human CD70 Cell line (CHO-K1) Human CD70 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22359201 Human CD33 Cell line (CHO-K1) Human CD33 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22375101 Human Napi2B Cell line(3T3) Human Napi2B 3T3 90%DMEM+10%FBS Lentiviral Cell pool 2* 6*10^6
B22416901 Human MICA Cell line (CHO-K1) Human MICA CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22428701 Human CD89 Cell line (CHO-K1) Human CD89 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22468801 Human CD8B Cell line (CHO-K1) Human CD8B CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Single clone 2* 6*10^6
B22468802 Human CMET Cell line (CHO-K1) Human CMET CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22468803 Human MSLN Cell line (CHO-K1) Human MSLN CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6
B22529803 Human TMPRSS4 Cell line (CHO-K1) Human TMPRSS4 CHO-K1 90%DMEM/F-12+10%FBS Lentiviral Cell pool 2* 6*10^6