PCR mycoplasma detection kit-客製化抗體-盟基生物科技股份有限公司

Mycoplasma (黴漿菌相關產品)

PCR mycoplasma detection kit

PCR mycoplasma detection kit

Mycoplasma (黴漿菌相關產品)

PCR mycoplasma detection kit

超省時又省力的黴漿菌檢測套組,只需要取細胞上清液進行 PCR 反應

一、產品介紹

黴漿菌是一種微生物,為沒有細胞壁的原核生物。大小 0.2-0.3 um,而且細胞型態不固定,因此有一定機率通過0.22 um 過濾膜,造成細胞汙染。由於黴漿菌沒有細胞壁,因此一般的抗生素無法清除黴漿菌,需要使用特別的抗生素 (請見產品 “Mycoplasma removal agent”)。黴漿菌為實驗室細胞培養常見的汙染源,與一般的細菌真菌汙染不同,黴漿菌汙染不會造成培養液混濁,也不會造成細胞死亡,因此不容易以顯微鏡和肉眼觀察。然而,黴漿菌的污染卻會造成細胞的改變,影響細胞所有的特性和行為,造成實驗結果上的誤差和錯誤。常見的現象有細胞生長速度緩慢、細胞轉染效率降低、細胞生產病毒效率降低以及培養液快速地變色。以高倍顯微鏡觀察下,可以看到奇怪的小黑點。
黴漿菌檢測的方法有很多,包括螢光檢測、菌盤培養法和抗體偵測,這些方法的缺點在於需要較多時間操作實驗,或需要特殊的儀器設備。Abm 研發的黴漿菌檢測套組使用上非常簡單,不須純化 DNA,直接以細胞上清液做傳統 PCR 反應,以電泳分析結果 (370-550 bp),可於二小時內完成反應。
NIH3T3 細胞有無汙染的構造,左圖為無汙染的細胞,表面光滑和平坦。右圖則為黴漿菌汙染的細胞,表面非常粗糙和有許多凸起。
對於這些令人頭痛的mycoplasma與病毒,盟基生物科技提供T-pro MycoClean spray,具有極佳的潔淨能力,例如:staphyhococcus aureus、streptococcus faecalis、Escherichia coli、Proteus mirabilis、Pseudomonas aeruginosa、Candida albicans、HBV、Papova-、Adeno-、Polio- and Vaccinia Viridae等。您只需要在欲清潔之表面噴上T-Pro MycoClean並於兩分鐘後擦拭或讓它在十分鐘內自然自然揮發掉即可。只需要一點點,就有不可思議的驚人效果。
 
 

二、產品特色

1. 操作超簡單:
直接取細胞培養的上清液做反應,以電泳分析,不需要純化 DNA (也可以使用純化後的 DNA 反應)。為傳統 PCR 操作,以電泳觀察,不須額外訓練。
Abm 黴漿菌檢測套組的流程示意圖,僅需取細胞培養的上清液做 PCR 反應即可。

2. 常規使用的抗生素 (penicillin, streptomycin, and gentamicin) 不影響實驗結果

3. 偵測範圍超廣:
可偵測200種以上黴漿菌
可被檢測到的黴漿菌菌種
verecundum fermentans M64 zalophi strain CSL 4296 hyopneumoniae J
verecundum strain ATCC 27862 fermentans JER zalophi strain CSL 5195 hyopneumoniae 168
gallisepticum strain XZA fermentans strain ATCC 49892 zalophi strain CSL 4296 hyopneumoniae strain ATCC 27714
gallisepticum strain JXB vulturii strain Gb‐V33 gateae strain KDC hyopneumoniae 7448
gallisepticum strain YMC vulturii strain Gb‐V33 gateae strain V‐1 hyopneumoniae 232
gallisepticum strain ML edwardii strain ATCC 23462 gateae strain ATCC 23392 zalophidermidis strain CSL 4779
gallisepticum strain WSA edwardii strain 04‐3436 canadense strain 466 pulmonis strain Ash (PG34)
gallisepticum strain JXA edwardii strain 04‐3440 canadense strain 275C pulmonis (strain UAB CTIP)
gallisepticum strain WSB flocculare canadense strain ATCC 29418 alligatoris strain ATCC 700619
gallisepticum strain XZC flocculare strain Ms42 canadense strain 466 crocodyli
gallisepticum strain F conjunctivae HRC/581T corogypsi strain BV1‐5 crocodyli MP145
gallisepticum strain MG‐HS conjunctivae strain Goat 655 corogypsi strain BV1‐1 crocodyli strain ATCC 51981
gallisepticum strain YMA conjunctivae strain Goat 655 equirhinis strain M432‐72 subdolum strain ATCC 29870
gallisepticum strain XA conjunctivae strain HRC 581 equirhinis strain ATCC 29420 phocicerebrale strain CSL 5195S2
gypis phocicerebrale ATCC 51405 canis strain ATCC 19525 phocicerebrale strain ATCC 51405
gypis strain ATCC 51370 phocicerebrale strain 1049 canis strain C3b phocicerebrale strain Cheryl
indiense hyorhinis GDL‐1 canis strain Skotti A bovis
gallinaceum hyorhinis clone V116‐1 hyopharyngis strain H36B‐F bovis strain HEK‐FDA
gallinaceum strain SA hyorhinis strain GDL hyopharyngis strain 538 Nasal bovis HB0801
gallinaceum strain ATCC 33550 hyorhinis ATCC:29052 auris bovis PG45 clone MU clone A2
gallinaceum strain 887 hyorhinis isolate LuSIV cloacale bovis strain Madison
gallinaceum strain 111R hyorhinis strain ATCC 29052 mustelae bovis Hubei‐1
gallinaceum strain 33F hyorhinis MCLD mustelae strain MX9 bovis strain 70‐213 (Del Giudice)
arginini sp. Phocoena C‐264‐GEN sp. 1220 bovis strain ATCC 25025
arginini strain EF‐Hungary sp. Phocoena C‐269 sp. 31848 gallinarum strain ATCC 15319
arginini strain ATCC 23243 agalactiae sp. 31948 gallinarum strain B2 (Dinter)
arginini clone V111‐1 agalactiae PG2 salivarium gallinarum ATCC 15319
sphenisci strain UCMJ agalactiae 5632 salivarium strain MP1166 phocirhinis strain CSL7520‐lung
sp. Mirounga ES2783_AG_GEN neurolyticum strain ATCC 19988 leonicaptivi strain ATCC 49890 phocirhinis strain CSL 7475‐4
sp. Mirounga ES2774‐NAS neurolyticum strain 10RN‐F‐3 anatis phocirhinis strain ATCC 49639
sp. Mirounga ES2806‐NAS neurolyticum strain T15 anatis strain K6193C collis strain ATCC 35278
sp. Mirounga ES2774‐NASSP4 neurolyticum strain 36CFA2 anatis strain K6193A californicum strain ST6
sp. Mirounga ES2714_AG_GEN agassizii strain PS6 felis strain ATCC 23391 californicum strain ATCC 33461
sp. Mirounga ES2805‐ORL agassizii strain Karina 09 Fr. Tortoise anseris spumans
pullorum falconis ATCC 51372 hyopharyngis buteonis strain Bb/T2g
pullorum strain ATCC 33553 lagogenitalium strain ATCC 700289 bovirhinis oxoniensis
gallopavonis strain WR1 ovipneumoniae strain ATCC 29419 simbae ATCC 49888 leopharyngis
gallopavonis strain ATCC 1197 columbinasale strain ATCC 33549 synoviae ATCC 25204 primatum strain HRC292
sp. CSL7489‐oral felifaucium strain PU synoviae 53 primatum strain I30
sp. CSL7529‐conjunctiva spermatophilum orale primatum strain ATCC 25948
sp. CSL7491‐lung spermatophilum strain AH159 orale strain MT‐4 molare strain ATCC 27746
sp. CSL7518‐lung spermatophilum strain 88(Jacobs) hyosynoviae strain ATCC 25591 lipofaciens strain ATCC 35015
dispar strain ATCC 27140 bovigenitalium strain MH 1113 bovoculi strain ATCC 29104 cricetuli
arthritidis strain 91021 bovigenitalium strain PG‐11 maculosum strain ATCC 19327 glycophilum
arthritidis strain ATCC 19611 bovigenitalium strain ATCC 14173 maculosum strain Skotti B citelli strain RG‐2C
arthritidis 158L3‐1 bovigenitalium strain ATCC 19852 elephantis strain ATCC 51980 citelli strain RG1D‐L
meleagridis equigenitalium strain ATCC 29869 columbinum citelli strain RG1D‐S
meleagridis strain ATCC 27764 iguanae strain 2327 columbinum strain FG295 citelli strain RG2C
iners strain PG30 testudineum strain ATCC 700618 caviae strain G122 citelli strain RG1D‐L
iners strain 89R‐D‐3 testudineum strain BH29 caviae strain ATCC 27108 columborale strain ATCC 29258
iners strain 1D‐E‐3 moatsii strain MK405 hominis ATCC 23114 columborale strain 18303
iners strain ATCC 15969 opalescens hominis strain ATCC 27545 sturni ATCC 51945


4. 偵測超靈敏:
只有10個黴漿菌/sample 也可以檢測到


5. 省時:
反應小於2小時

6. 附上正控制組:
為包含黴漿菌 gDNA 的載體,確保實驗步驟正確以及無 PCR inhibitor 干擾

 

三、產品資訊

貨號 品名 包裝
G238 PCR Mycoplasma Detection kit 100 reactions
 


四、相關產品資訊

貨號 品名 包裝
JT90-R002 T-pro MycoClean Spray 500 ml
093050044 Mycoplasma Removal Agent (MRA) 5 ml