PMA dye & PMA-PCR kits for bacterial viability-客製化抗體-盟基生物科技股份有限公司

PMA dye & PMA-PCR kits for bacterial viability

PMA dye & PMA-PCR kits for bacterial viability

Bacteria

PMA dye & PMA-PCR kits for bacterial viability

介紹

對於部分的應用與研究,例如:食物安全檢測、飲用水的品質控制、環境微生物檢測以及醫療診斷等,偵測樣本中的活菌,試劑的選擇性與敏感性是相當重要的,傳統的方法為培養細菌,但相當耗時且不夠靈敏;雖然可以通過PCR的方式得到大量細菌DNA,其優點為快速與高靈敏度,然而,這樣的方式卻不能夠區分樣本中的死細胞與活細胞,造成PCR的結果很難給予準確的解釋。Biotiu的PMAxx克服了這樣的難題,在進行PCR前將樣品先以PMAxx處理,因此可選擇性專一的偵測活細胞。

PMAxx是一種光反應染劑,與DNA具有高親和力,由於此染劑不具有通透膜性,僅在可見光的暴露下會進入至死細胞中並嵌在雙股DNA中形成共價鍵結之修飾(圖一),進而達到抑制死細胞之PCR擴增反應(圖二)。於Real-Time PCR反應中,相較於活細胞,死細胞擴增現象被延遲並且有較高的CT值(圖三)。於混合型族群,qPCR可定量細胞存活率。

產品特色

方便快速 - 只需在收細胞時加入染劑即可
超高專一性 - 只會進入膜有受損的細菌中
超高靈敏度 - 比EMA與PMA的靈敏度更高
提高實驗組與控制組之間的差異 - 只與死細胞 DNA 結合,可提高 qPCR實驗組與控制組之間的差異

傳統與光反應核酸染劑之比較


比較不同光反應核酸染劑


訂購資訊

其他相關產品訂購資訊



若對Biotium的PMA系列產品有任何疑問,請來電洽詢盟基生物科技或當區業務。盟基生物科技的專業服務團隊提供了研究人員完整的銷售前與銷售後的技術資源,讓您於研究上無後顧之憂。

PMAxx dye

產品說明

PMAxx染劑為一種光反應染劑,為進階升級版的PMA染劑,可更有效的從樣本中清除死細胞的DNA。PMAxx的操作步驟與PMA皆相同,

For experienced users of PMA™, PMAxx™ can be used in your current PMA™-PCR protocol. In fact, because of its high activity, you may be able to use PMAxx™ at a lower concentration. PMAxx™ is also compatible with our PMA-Lite™ (see below) and PMA™ Enhancer for Gram-Negative Bacteria (see next page).

圖 PMAxx可藉由利用PCR可更有效率的區分死細胞與活細胞
活細胞或死掉的細胞以25uM PMA或PMAxx染色後利用PMA光分解儀進行曝光

Figure 3. PMAxx™ allows for better discrimination of live and dead bacteria in viability PCR. (A) Live or heat-killed Bacillus subtilis were treated with 25 uM PMA™ or PMAxx™, followed by exposure with the PMA-Lite™ and DNA purification. Fast EvaGreen® qPCR Master Mix was used to amplify a 500-bp fragment of B. subtilis DNA. Dead cells treated with PMAxx™ showed a significant further delay (>7 Ct) compared to dead cells treated with PMA™. (B) Each of the indicated bacteria were treated with 25 uM PMA™ or PMAxx™, as described in (A). dCt values were calculated by subtracting the Ct without dye from the Ct with dye. ddCt values were calculated by subtracting the dCt for live cells from the dCt for dead cells. PMAxx™ improved live/dead discrimination in all bacteria tested, with improvements in ddCt ranging from 3 to 12.