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Cell line (穩定細胞株)

GPCR, Immune Checkpoint Cell line

GPCR, Immune Checkpoint Cell line

Cell line (穩定細胞株)

GPCR, Immune Checkpoint Cell line

GPCR與Immune checkpoint 功能性細胞株

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一、產品介紹

Genscript 提供穩定表現 GPCR 或免疫相關蛋白的細胞株。GPCR (G protein-coupled receptors) 為常見的訊息傳遞路徑的受體，參與許多功能，包括神經、肌肉和生長等等，因此很多藥物是透過結合 GPCR (抑制或活化) 來達到療效。為了大量篩選藥物，必須先建構一個穩定表現 GPCR 的細胞株。因此，Genscript 建構的細胞株可以大量節省時間和人力。
Genscript 提供的免疫相關蛋白表現的細胞株包含三類，第一類是 Immune checkpoint for binding assay (免疫抑制蛋白)，細胞表現免疫抑制的蛋白，用來針對癌症免疫療法的研究和藥物篩選。第二類是 Immune activation for functional assay (免疫活化蛋白)，細胞表現免疫活化的蛋白，給予相對應的 ligand 可以活化細胞，促使 IL-8 的分泌。第三類是 Fc Receptor，細胞表現 Fc receptor，可以結合抗體的 Fc region，可以用來研究抗體的結合 (binding assay)。三類不同的細胞株可以應用在不同層面，可以拿來測試結合力，也可以拿來做篩選藥物的平臺。Genscript 提供的這些細胞株都能夠為實驗者省下大量時間和人力。
盟基也有提供全客製化的細胞株建構服務，歡迎來電詢問。

 

二、產品特色

GenScript 提供的特殊蛋白表現細胞株 (兩管，1x106 細胞/管)：

1. 皆經過 FACS 確認蛋白穩定表現：
經過超過15代以上培養，以 FACS 確認，因此確認蛋白質穩定表現在細胞膜上。
以 FACS 分析 CHOK1 (紅) 和 CHO-K1/PD-1 (藍) 細胞的 PD-1 表現量。 以 FACS 分析 GS-H2 (紅) 和 GS-H2/CD27 (藍) 細胞的 CD27 表現量。
 

2. 節省篩選藥物前置作業時間和人力：
篩選藥物前必須先建構穩定表現蛋白的細胞株，因此 Genscript 建構的細胞株可以作為篩選藥物平臺，省下大量細胞建構時間和人力。

3. GPCR 表現細胞株和免疫活化蛋白表現細胞株經過功能性確認：
GPCR 表現細胞株給予相對應的 ligand 後，路徑皆能夠活化，促使鈣離子的流動。
免疫活化蛋白表現細胞株給予相對應 ligand 後，皆能夠使免疫細胞活化，促使 IL-8 的分泌。
CHO-K1/M1 和 CHO-K1 細胞給予 M1 receptor 活化物 carbachol，並以 calcium-4 螢光染劑偵測鈣離子，透過 FlexStation 儀器紀錄。以相關螢光數值 [relative fluorescent units (RFU)] 對 log [carbachol 濃度] 做圖。 功能性分析確認，給予 GS-H2/CD27 細胞 CD70，檢測活化後產生的 IL-8。
 
 
三、產品資訊
表一：GPCR 穩定表現細胞株訂購資訊
貨號 產品名稱 家族 受體
M00330 CHO-K1/5-HT1A/Gα15 5-Hydroxytryptamine receptor 5-HT1A/HTR1A
M00251 CHO-K1/5-HT2A 5-HT2A/HTR2A
M00249 CHO-K1/5-HT2B 5-HT2B/HTR2B
M00253 CHO-K1/5-HT2C 5-HT2C/HTR2C
M00319 HEK 293/5-HT2C 5-HT2C/HTR2C
M00444 CHO-K1/5-HT4/Gα15 HTR4
M00185 CHO-K1/M1 Acetylcholine receptor (muscarinic) M1/CHRM1
M00258 CHO-K1/M2/Gα15 M2/CHRM2
M00259 CHO-K1/M3 M3/CHRM3
M00238 CHO-K1/M4/Gα15 M4/CHRM4
M00186 CHO-K1/M5 M5/CHRM5
M00324 CHO-K1/ADORA1/Gα15 Adenosine receptor ADORA1
M00255 HEK293/ADORA1/Gα15 ADORA1
M00246 CHO-K1/ADORA2A/Gα15 ADORA2a
M00575 CHO-K1/mouse ADORA2A ADORA2a
M00329 CHO-K1/ADORA2B/Gα15 ADORA2B
M00464 CHO-K1/ADORA3/Gα15 ADORA3
M00260 CHO-K1/ADRA1B Adrenoceptors ADRA1B
M00340 CHO-K1/ADRA1D ADRA1D
M00281 CHO-K1/ADRA2A/Gα15 ADRA2A
M00454 CHO-K1/ADRA2B/Gqi5 ADRA2B
M00252 CHO-K1/ADRA2C/Gα15 ADRA2C
M00269 CHO-K1/ADRB1/Gα15 ADRB1
M00225 CHO-K1/ADRA1A ADRA1A
M00458 HEK 293/AT1 Angiotensin receptor AT1
M00448 HEK293T/AT1 AT1
M00245 CHO-K1/AGTRL1/Gα15 Apelin receptor AGTRL1
M00432 CHO-K1/GPBAR1 Bile acid receptor GPBAR1
M00254 CHO-K1/BB1 Bombesin receptor BB1
M00182 CHO-K1/BB2 BB2/GRPR
M00183 CHO-K1/BB3 BB3/BRS3
M00184 CHO-K1/B2/Gα15 Bradykinin receptor B2
M00557 CHO-K1/Gα15/AMY1   Calcitonin AMY1
M00558 CHO-K1/Gα15/AMY2   AMY2
M00559 CHO-K1/Gα15/AMY3    AMY3
M00320 CHO-K1/CT/Gα15 Calcitonin receptor CALCR/CT
M00299 CHO-K1/CB1/Gα15 Cannabinoid receptor CNR1/CB1
M00433 CHO-K1/CB2 CB2
M00301 CHO-K1/CMKLR1/Gα15 Chemerin receptor CMKLR1
M00522 CHO-K1/CCR1/Gα15    Chemokine receptor CCR1
M00523 CHO-K1/CCR5/Gα15    CCR5
M00524 CHO-K1/CXCR1/Gα15    CXCR1/IL8RA
M00525 CHO-K1/CXCR3/Gα15    CXCR3
M00556 CHO-K1/CXCR4/Gα15    CXCR4
M00526 CHO-K1/CXCR5/Gα15    CXCR5/BLR1
M00271 CHO-K1/CCKA/Gα15 Cholecystokinin receptor CCKA/CCKAR
M00154 CHO-K1/CCKB CCKB/CCKBR
M00439 CHO-K1/GPR68/Gα15 Class A orphan GPR68/OGR1
M00425 CHO-K1/MRGPRX2 MRGPRX2
M00327 CHO-K1/C5a/Gα15 Complement peptide receptor C5a/C5AR1
M00343 CHO-K1/CRF1/Gα15 Corticotropin releasing factor receptor CRF1/CRHR1
M00208 CHO-K1/CRF2/Gα15 CRF2
M00152 CHO-K1/D2/Gα15 Dopamine receptor D2
M00322 CHO-K1/D5/Gα15 D5
M00247 CHO-K1/D1 D1/DRD1
M00274 CHO-K1/D2s/Gα15 D2(Short)
M00149 CHO-K1/EDNRA Endothelin receptor ETA/EDNRA
M00150 CHO-K1/EDNRB ETB/EDNRB
M00429 CHO-K1/FPR1/Gα15 Formylpeptide receptor FPR1
M00437 CHO-K1/FFA2/Gα15 Free fatty acid receptor FFA2
M00273 CHO-K1/FFA1/Gα15 GPR40/FFA1
M00277 CHO-K1/GALR1/Gα15 Galanin receptor GALR1
M00209 CHO-K1/GALR2 GALR2
M00314 HEK293/GHRH/Gα15 Glucagon receptor GHRHR
M00451 CHO-K1/GLP1R/Gα15 GLP1R
M00307 CHO-K1/GLP2R/Gα15 GLP2R
M00345 CHO-K1/GCGR/Gα15 GCGR
M00422 HEK 293/GCGR/Gα15 GCGR
M00486 CHO-K1/GIP/Gα15 GIPR
M00426 CHO-K1/GNRHR/Gα15 Gonadotropin releasing hormone receptor GNRHR
M00189 CHO-K1/GHSR Growth hormone releaseing peptides receptor GHSR
M00131 HEK293/H1 Histamine receptor H1/HRH1
M00306 CHO-K1/H2/Gα15 H2/HRH2
M00331 CHO-K1/H3/Gα15 H3/HRH3
M00289 CHO-K1/H4/Gα15 H4/HRH4
M00428 CHO-K1/NIACR1/Gα15 Hydroxycarboxlic acid receptor GPR109A
M00190 CHO-K1/KiSS1 Kisspeptin receptor KiSS1/GPR54
M00305 CHO-K1/FPRL1/Gα15 Leukotriene receptor FPRL1
M00462 CHO-K1/CysLT1/Gα15 CysLT1
M00435 CHO-K1/LTB4/Gα15 LTB4R
M00463 RH7777/EDG4 Lysophospholipid receptor EDG4/LAP2
M00211 HEK293/MCH1 Melanin concentrating hormone receptor MCH1/GPR24
M00280 CHO-K1/MCH2 MCH2/GPR145
M00336 CHO-K1/MC2/Gα15 Melanocortin receptor MC2
M00242 CHO-K1/MC3/Gα15 MC3
M00272 CHO-K1/MC4/Gα15 MC4
M00243 CHO-K1/MC5/Gα15 MC5
M00424 CHO-K1/MT1/Gα15 Melatonin receptor MTNR1A
M00312 CHO-K1/MT2/Gα15 MT2/MTNR1B
M00427 CHO-K1/GRM2/Gα15 Metabropic glutamate receptor mGlu2/GRM2
M00347 CHO-K1/MTLR/Gα15 Motilin receptor MTLR/MLNR
M00244 HEK293T/NMU2 Neuromedin receptor NMU2
M00191 CHO-K1/NMU1 NMU1/GPR66
M00344 CHO-K1/NPS1b/Gα15 Neuropeptide S receptor NPS1b
M00338 CHO-K1/NPS1a/Gα15 NPSR1
M00460 CHO-K1/NPY2/Gqi5 Neuropeptide Y receptor NPY2
M00237 CHO-K1/NPY4/Gα15 NPY4R
M00194 CHO-K1/NTS1 Neurotensin receptor NTS1/NT1
M00323 CHO-K1/OPRD1/Gα15 Opioid & opioid-like receptor OPRD1
M00290 CHO-K1/OPRK1/Gα15 OPRK1
M00304 CHO-K1/OPRM1/Gα15 OPRM1
M00447 CHO-K1/OPRL1/Gα15 OPRL1
M00224 CHO-K1/OX1 Orexin receptor OX1
M00316 CHO-K1/OX2 OX2
M00250 1321N1/P2Y1 P2Y receptor P2Y1
M00318 1321N1/P2Y2 P2Y2
M00297 1321N1/P2Y4 P2Y4
M00346 1321N1/P2Y6 P2Y6
M00298 1321N1/P2Y11 P2Y11
M00261 1321N1/P2Y12/Gα15 P2Y12
M00315 CHO-K1/PTH1/Gα15 Parathyroid hormone receptor PTH1/PTHR1
M00321 CHO-K1/GPR103/Gα15 Peptide P518 receptor QRFP/GPR103
M00262 CHO-K1/PTAFR Platelet activating factor receptor PAFR/PTAFR
M00341 CHO-K1/PRLHR Prolactin releasing peptide receptor PRLHR
M00436 CHO-K1/DP2/Gα15 Prostanoid receptor DP2
M00228 HEK293/EP1 EP1/PTGER1
M00311 CHO-K1/EP2/Gα15 EP2/PTGER2
M00155 HEK293/FP/Gα15 FP/PTGFR
M00310 CHO-K1/IP1/Gα15 IP1/PTGIR
M00309 HEK293/TP TP/TBXA2R
M00453 CHO-K1/DP1/Gα15 PTGDR/DP1
M00446 CHO-K1/PAR2/Gα15 Proteinase-activated receptor PAR2
M00206 CHO-K1/PAR4 PAR4/F2RL3
M00473 CHO-K1/SST1/Gqi5 Somatostatin receptor SST1
M00325 CHO-K1/SST2/Gα15 SST2/SSTR2
M00285 CHO-K1/SST3/Gα15 SST3
M00334 CHO-K1/SST4/Gα15 SST4/SSTR4
M00286 CHO-K1/SST5/Gα15 SST5/SSTR5
M00199 CHO-K1/NK1 Tachykinin receptor NK1/TACR1
M00200 CHO-K1/NK2 NK2/TACR2
M00201 CHO-K1/NK3 NK3/TACR3
M00202 CHO-K1/TRH1 Thyrtropin releasing hormone receptor TRH1/TRHR
M00339 CHO-K1/UT2/Gα15 Urotensin receptor UTR2
M00195 CHO-K1/OXTR Vasopressin and oxytocin receptor OT/OXTR
M00205 CHO-K1/V1B V1B
M00170 CHO-K1/V2/Gα15 V2
M00203 CHO-K1/PAC1/Gα15 VIP and PACAP receptor PAC1
M00226 CHO-K1/VPAC2/Gα15 VPAC2
M00342 CHO-K1/VPAC1/Gα15 VPAC1/VIP1
M00554 HEK293/Gα15 Control \
M00257 CHO-K1/Gα15 \
M00455 CHO-K1/Gqi577 \
       
表二：Immune checkpoint for binding assay (免疫抑制蛋白) 細胞株訂購資訊  
貨號 產品名稱 受體
M00538 CHO-K1/4-1BB  4-1BB
M00568 CHO-K1/mouse 4-1BB
M00569 CHO-K1/cyno 4-1BB
M00671 CHO-K1/B7-H2 B7-H2
M00536 CHO-K1/B7-H3 B7-H3
M00537 CHO-K1/B7-H4 B7-H4
M00595 CHO-K1/BDCA2 BDCA2
M00596 CHO-K1/Cyno BDCA2
M00593 CHO-K1/BDCA2 and FcER1G
M00594 CHO-K1/ Cyno BDCA2 and FcER1G
M00534 CHO-K1/BTLA BTLA
M00591 CHO-K1/CD155 CD155
M00643 CHO-K1/Cyno CD155
M00642 CHO-K1/Mouse CD155
M00541 CHO-K1/CD200 R1 CD200 R1
M00553 CHO-K1/human CD38 CD38
M00636 CHO-K1/mouse CD38
M00581 CHO-K1/CD47 CD47
M00631 CHO-K1/Mouse CD47
M00632 CHO-K1/Cyno CD47
M00590 CHO-K1/CD112 CD112
M00545 CHO-K1/CD80 CD80
M00592 CHO-K1/ITGAE CD103
M00530 CHO-K1/CTLA4 CTLA4
M00570 CHO-K1/mouse CTLA4
M00571 CHO-K1/cyno CTLA4
M00539 CHO-K1/GITR GITR
M00646 CHO-K1/HVEM HVEM
M00647 CHO-K1/mouse HVEM
M00648 CHO-K1/Cyno HVEM
M00649 CHO-K1/ICOS ICOS
M00650 CHO-K1/KIR KIR
M00532 CHO-K1/Lag3  Lag3
M00561 CHO-K1/OX-40 OX-40
M00529 CHO-K1/PD-1 PD-1
M00552 CHO-K1/mouse PD-1
M00572 CHO-K1/cyno PD-1
M00630 Daudi/PD-1
M00544 HEK293/PD-L1 PD-L1
M00543 CHO-K1/PD-L1
M00567 CHO-K1/mouse PD-L1
M00573 CHO-K1/cyno PD-L1 
M00627 CHO-K1/PD-L2 PD-L2
M00629 CHO-K1/cyno PD-L2
M00533 CHO-K1/VISTA  VISTA
M00633 CHO-K1/Mouse VISTA
M00634 CHO-K1/Cyno VISTA
M00542 CHO-K1/TIGIT TIGIT
M00585 CHO-K1/mouse TIGIT 
M00531 CHO-K1/Tim3 Tim3
 
表三：Immune activation for functional assay (免疫活化蛋白) 細胞株訂購資訊  
貨號 產品名稱 受體
M00610 HT1080/CD27 CD27
M00611 Jurkat/CD28 CD28
M00606 HT1080/CD40 CD40
M00614 CHO-K1/CD80 CD80
M00607 HT1080/GITR GITR
M00608 HT1080/OX40  OX40
M00609 HT1080/4-1BB  4-1BB
 
表四：Fc Receptor 細胞株訂購資訊
貨號 產品名稱 受體
M00586 CHO-K1/CD16A 158F CD16A
M00597 CHO-K1/CD16A 158V
M00602 CHO-K1/CD16B CD16B
M00598 CHO-K1/CD32A 131His CD32A
M00600 CHO-K1/CD32B 232Thr CD32B
M00601 CHO-K1/CD32C 13Gln CD32C
M00588 CHO-K1/CD64 CD64
M00603 CHO-K1/FcRn FcRn

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