映照您的Data光芒,帶來全新的螢光體驗!-盟基生物科技股份有限公司

客製化抗體-盟基生物科技股份有限公司

促銷活動

映照您的Data光芒,帶來全新的螢光體驗!

映照您的Data光芒,帶來全新的螢光體驗!
活動時間:即日起至9月底

優惠促銷方案請洽當區業務代表,或來電洽詢。


一、跨時代的 CF® Dyes 螢光二抗

應用:免疫螢光染色、Flow cytometry、螢光 Western-blotting
針對市面螢光染劑大幅改良,有以下優點:
1. 超過30種螢光選擇
2. 螢光強度更高:優於 Alexa Fluor 染劑
3. 穩定性更高:長時間照射不失活性,更適合顯微鏡觀察。
4. 背景值更低:CF® dye 負電荷較 Alexa Fluor®, DyLight® and IRDyes®少,因此染劑對細胞的非專一性吸附較少,亦不影響抗體等電點
5. 酸鹼耐受性更高:耐受 pH 2至pH 11



二、輕輕壓一滴, 完成螢光二抗實驗
Drop-n-stain
應用:免疫螢光染色、Flow cytometry、螢光 Western-blotting
訂購資訊:
貨號 品名 包裝 螢光
20950 Drop-n-Stain™ CF®488A Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed 5 mL CF488A
20951 Drop-n-Stain™ CF®594 Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed CF594
20952 Drop-n-Stain™ CF®488A Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed CF488A
20953 Drop-n-Stain™ CF®594 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed CF594
20954 Drop-n-Stain™ CF®488A Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed CF488A
20955 Drop-n-Stain™ CF®594 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed CF594
20956 Drop-n-Stain™ CF®488A Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed CF488A
20957 Drop-n-Stain™ CF®594 Goat Anti-Mouse IgG (H+L), highly corss-adsorbed CF594
20962 Drop-n-Stain™ CF®640R Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed CF640R
20963 Drop-n-Stain™ CF®640R Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed CF640R
20964 Drop-n-Stain™ CF®640R Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed CF640R
20965 Drop-n-Stain™ CF®640R Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed CF640R
20966 Drop-n-Stain™ CF®543 Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed CF543
20967 Drop-n-Stain™ CF®543 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed CF543
20968 Drop-n-Stain™ CF®543 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed CF543
20969 Drop-n-Stain™ CF®543 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed CF543


三、抗體螢光標記套組
Mix-n-Stain™ CF® Dye Antibody Labeling Kits

非用不可的五大理由!
1. 大幅減少螢光免疫染色時間
2. 共價鍵結非常穩定:多螢光實驗中螢光染劑不會擴散或轉移到其他抗體上,鍵結完的抗體可以於4°C 保存半年
3. 反應只要30分鐘 (酵素需要較長時間)
4. 不需要純化且背景值低:CF dye 水溶性超高,未鍵結的 CF dye 容易洗掉
5. 可以忍受多種干擾物:優化後的試劑可以忍受  glycine、Tris、glycerol、sodium azide 和其他蛋白質



訂購資訊一覽表
Label Dye Ex/Em Catalog for Labeling
5-20 ug antibody 20-50 ug antibody 50-100 ug antibody 1mg antibody
CF®350 347/448 nm 92270 92250 92230 92420
CF®405S 404/431 nm 92271 92251 92231 92421
CF®405M 408/452 nm 92272 92252 92232 92404
CF®405L 395/545 nm 92303 92304 92305 x
CF®430 426/498 nm 92316 92317 92318 x
CF®440 440/515 nm 92319 92320 92321 x
CF®450 405/460 nm 92322 92323 92324 x
CF®488A 490/515 nm 92273 92253 92233 92405
CF®514 516/548 nm 92331 92332 92333 x
CF®532 527/558 nm 92289 92290 92291 x
CF®543 541/560 nm 92287 92267 92247 x
CF®555 555/565 nm 92274 92254 92234 92406
CF®568 562/583 nm 92275 92255 92235 92407
CF®570 568/591 nm 92334 92335 92336 x
CF®583 583/606 nm 92237 92238 92239 x
CF®594 593/614 nm 92276 92256 92236 92408
CF®633 630/650 nm 92277 92257 92237 92409
CF®640R 642/662 nm 92278 92258 92245 x
CF®647 650/665 nm 92279 92259 92238 92410
CF®660C 667/685 nm 92280 92260 92239 x
CF®660R 663/682 nm 92281 92261 92243 x
CF®680 681/698 nm 92282 92262 92240 92422
CF®680R 680/701 nm 92283 92263 92246 x
CF®700 695/720 nm 92425 92426 92427 x
CF®750 755/777 nm 92284 92264 92241 92423
CF®770 770/797 nm 92285 92265 92242 92424
CF®790 784/806 nm 92288 92268 92248 x
CF®800 797/816 nm 92428 92429 92430 x
CF®820 822/835 nm 92431 92432 92433 x
Cyanine 555 555/565 nm 92412 92413 92414 92415
Cyanine 647 650/665 nm 92416 92417 92418 92419
FITC 494/518 nm 92294 92295 92296 92411
Biotin x 92286 92266 92244 x
DIG x 92328 92329 92330 x
DNP x 92325 92326 92327 x
Alkaline Phosphatase x x 92314
(25-50 ug)
92315 x
Glucose Oxidase x x 92312
(25-50 ug)
92313 x
HRP x 92300
(10-20 ug)
92301
(25-50 ug)
92302 92437


四、螢光染色封片膠
應用:
螢光免疫染色實驗中,以抗體標定上螢光後,加上 mounting medium 覆蓋住組織或細胞,才蓋上蓋玻片,最後以螢光顯微鏡觀察螢光。可以避免樣本水分流失,以及保護螢光延長顯微鏡觀察和樣本保存時間。


優點:
1. 樣本 4°C 保存一年以上
2. 螢光保護效果優於他牌
3. 所有螢光皆有保護效果,包括Cyanine-based 的螢光 (Cy3, Cy5)


 
螢光保護效果優於他牌
檢測四種不同 mounting medium 條件 (三家廠商 + PBS) 在螢光顯微鏡下對螢光 FITC 的保護效果。(FITC staining in HeLa cells) 以各自的初始時間 (t0) 為基準 (100%),在螢光顯微鏡下照射120秒,每15秒觀察螢光衰退情形。Biotium Everbrite 比起 SlowFade Gold 螢光保護效果更好。


Mounting medium 訂購資訊
應用 貨號 產品名稱 DAPI 包裝
Wet-set #23001 EverBrite™ Mounting Medium W/O DAPI 10 mL
#23002 With DAPI 10 mL
Harden (24 hours) #23003 EverBrite™ Hardset Mounting Medium W/O DAPI 10 mL
#23004 With DAPI 10 mL
Wet-set (dropper bottle) #23008-T Drop-n-Stain EverBrite™ Mounting Medium W/O DAPI 2 mL
#23008 10 mL
#23009-T With DAPI 2 mL
#23009 10 mL


其他相關產品
應用 貨號 產品名稱 包裝
玻片染色封片 #23005 CoverGrip™ Coverslip Sealant 15 mL
#23005-1 100 mL
玻片染色實驗中隔出疏水性區域 #22005 SuperHT PAP Pen Mini 2.5 mm
#22006 SuperHT PAP Pen Regular 4 mm
細胞核染色 #40043 DAPI in H2O, 10 mg/mL 1 mL
#40046 Hoechst 33342, 10 mg/mL in H2O 10 mL
細胞打洞 #22016 Permeabilization Buffer 100 mL
#22017 Permeabilization and Blocking Buffer 100 mL

 
五、螢光實驗降低背景值 [TrueBlack 系列]

螢光 WB Blocking 試劑:TrueBlack® WB Blocking Buffer Kit
應用:
螢光 Western-blotting 降低背景值 (HRP 冷光不適用)

優點:
1. 同時降低抗體 & 螢光染劑造成的非專一性背景值
2. PVDF & NC 轉漬膜皆可使用
3. 非動物性試劑,適用所有抗體


免疫螢光染色 IF Blocking 試劑:TrueBlack® IF Background Suppressor System (Permeabilizing)
應用:
免疫螢光實驗中,除了抗體會有非專一性吸附,抗體上標記的螢光也會因為負電荷而吸附在細胞組織上,造成高背景值。此產品可以同時降低兩者造成的背景值。

優點:
1. 同時提供 blocking, permeabilization & 抗體稀釋
2. 非動物性 blocking 試劑,適用所有二抗


去除組織自體螢光:TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO
應用:
組織中的 lipofuscin/ECM 會有自體螢光,造成背景值而影響實驗觀察,此產品可以消除自體螢光。

優點:
1. 試劑本身幾乎沒有螢光,far-red 背景值更低
2. 全新配方溶於水溶液 (PBS),可反應更長時間,避免酒精造成樣本萎縮
3. 可於染色前或染色後使用

產品資訊
貨號 品名 包裝
23013-T TrueBlack® WB Blocking Buffer Kit 10 preps
23013 50 preps
23012-T TrueBlack® IF Background Suppressor System 20 assays
23012 200 assays
23014-T TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO 50uL
23014 250uL